Lefty Randy Wolf will move into the rotation, and the veteran will make his first start on Sunday against the Brewers at Marlins Park.
DeSclafani was promoted from Double-A Jacksonville to be an immediate fill-in after Jose Fernandez went down with a right elbow injury that required Tommy John surgery.
Wolf was signed shortly after the Fernandez injury to be a veteran presence in the rotation.
DeSclafani made two starts, collecting a win at Dodger Stadium in his Major League debut, giving up two runs in six innings with seven strikeouts. But on Tuesday, in his first start at Marlins Park, the 24-year-old worked 5 1/3 innings, and he gave up five earned runs.
At Triple-A, DeSclafani will join the rotation, and if he is promoted back to the big leagues this year, it could be as a reliever.
Joe Frisaro is a reporter for MLB.com. He writes a blog, called The Fish Pond. Follow him on Twitter