As a precaution, Infante was held out of the starting lineup in Wednesday's 9-1 victory over the Cubs and will not start Thursday in the series finale.
The Marlins are hopeful the veteran second baseman returns as early as Friday at Washington.
"He feels a little tight. He hurt it a little bit yesterday," manager Ozzie Guillen said. "It wasn't good enough for him to go out and play."
Infante said he aggravated his groin on his double Tuesday night. He remained in the game, but didn't feel right on Wednesday.
Donnie Murphy is filling in at second base until Infante is ready to start. Murphy went 2-for-3 with a home run and three RBIs on Wednesday.
Infante initially tweaked his groin last Friday against the Astros. He stayed in the game, but he was given Saturday off.
On Wednesday, the second baseman took the day off from taking batting practice or fielding.
"In a couple of days, I should be good," Infante said.
Infante has been one of the Marlins' hottest hitters, batting .333 with four doubles, four home runs and six RBIs.
"It's a little bit precautionary to make sure that he's fine for the next series," Guillen said.