As Guillen crossed the field to the visitors' clubhouse, which requires a walk in front of the third-base side home dugout and right in front of Valentine, the Sox's skipper gave a wave.
Guillen said afterward he did not see it, and his reaction may not have been tame if he had.
Valentine, who has not been ejected yet since joining the Red Sox, indicated after the game that he believes ejections in Spring Training are best avoided.
"I certainly hope not. Probably," Valentine said when asked if he had ever been ejected during a Spring Training contest. "Ozzie was telling the first-base umpire it wasn't his call, because the ball was in front of the bag, and he didn't want to hear that."
Asked if he said anything to Guillen, Valentine's response said his only words were, "See ya."
Guillen leaned on the notion that his players needed to be protected, whether the games count or not.
"Little excitement, set the tone. Protecting my players," Guillen said. "That's it. My first-base coach told me the ball was fair, I'm going to believe him.
"What's the difference between Spring Training and during the season? I do my job, the umpires have to do their job, the players have to do their job. My job is to protect the players. I don't see this game as different than others."
Valentine was also asked about Guillen's ejection during his in-game interview with ESPN, and reportedly Valentine joked he was waving goodbye to Guillen because the two had not had a chance to say hello before the game.
Tiffee was a late addition to Monday's lineup because of Greg Dobbs' bothersome left hamstring. When Tiffee batted in the sixth against Andrew Bailey, the Marlins trailed 3-2, and there were two runners in scoring position. A fair call likely would have allowed Miami to tie the game at 3. Tiffee hit into a fielder's-choice groundout and the Marlins did not score in the inning.
"I don't mind that in a manager, he's got your back," Tiffee said. "That's about it."
Said Guillen: "I get ejected protecting my players, I can sleep at night."
Valentine was reportedly a candidate to become the Marlins' manager twice, in 2010 and again during the most recent go-round in '11, when Guillen was hired.